Table 1. Genetic analysis of C. neoformans heterozygous mutants for the MATα and MATa ribosomal proteins Rpl39 and Rpl22.
Genotype | Strain | Protein function | Germinated basidiospores/ dissected basidiospores | NAT-R progeny/ basidiospore germinated | Phenotype |
---|---|---|---|---|---|
RPL39α/rpl39aΔ | GI229 | Large Subunit Ribosomal Protein L39 | 31/80 – 38.7% | 0/31 | Inviable |
RPL39a/rpl39αΔ | GI233 | 29/70 – 41.4% | 0/29 | Inviable | |
RPL22α/rpl22aΔ | GI56 | Large Subunit Ribosomal Protein L22 | 23/63 – 36.5% | 1/23a | Inviable |
RPL22a/rpl22αΔ | GI81 | 28/72 – 38.8% | 0/28 | Inviable |
The NAT-R progeny obtained is likely aneuploid for chromosome 5, because PCR confirmed the presence of the RPL22α allele and the mutated copy of the RPL22a.