Table 2. Genetic analysis of C. neoformans complementing strains of heterozygous mutants RPL22a/rpl22αΔ and RPL22α/rpl22aΔ.
| Strain | Genotype | Germinated basidiospores/ dissected basidiospores | NAT-R | NEO-R | NAT-R + NEO-R | MATa | MATα | MATa - MATα | Phenotype |
|---|---|---|---|---|---|---|---|---|---|
| GI86 | RPL22a/rpl22αΔNAT + RPL22α-NEO | 19/70 | 0 | 8 | 4b | 15 | 4b | 0 | Viable |
| GI150 | RPL22a/rpl22αΔNAT + RPL22a-NEO | NA | NA | NA | NA | NA | NA | NA | NA |
| GI83 | RPL22a/rpl22αΔNAT + empty NEO vector | 11/63 | 0 | 7 | 0 | 11 | 0 | 0 | Inviable |
| GI151 | RPL22α/rpl22aΔNAT + RPL22a-NEO | 19/63 | 0 | 6 | 3b | 3b | 16 | 0 | Viable |
| GI102 | RPL22α/rpl22aΔNAT + RPL22α-NEO | 17/58 | 2a | 0 | 0 | 0 | 15 | 2 | Inviable |
| GI154 | RPL22α/rpl22aΔNAT + RPL22α-NEO | 20/69 | 1a | 12 | 3 | 0 | 16 | 4 | Inviable |
| GI104 | RPL22α/rpl22aΔNAT + empty NEO vector | 10/57 | 0 | 8 | 1 | 0 | 9 | 1 | Inviable |
a
NAT-R progeny with 1 extra copy of RPL22α.
b
Progeny with markers that indicate success of the complementation experiment.
NA, Not available (strain GI150 did not produce basidiospores for analysis).