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. 2019 Dec 30;32(3):666–682. doi: 10.1105/tpc.19.00157

Table 1. Accumulation of Exogenous Metabolites in Leaf Discs.

Compound Metabolite Level Ratio (Exposed/Nonexposed ± se)
Amino acids
 Gly 5.2 ± 1.1a
 Ala 5.6 ± 0.2a
 Val 50.4 ± 5.1a
 Leu 105.6 ± 5.4a
 Ile 98.9 ± 9.1a
 Met 47.8 ± 4.3a
 Phe 39.9 ± 3.9a
 Pro 39.9 ± 4.2a
 Ser 15.0 ± 1.6a
 Thr 13.2 ± 1.0a
 Asn 6.0 ± 2.0a
 Gln 8.8 ± 0.9a
 Glu 1.4 ± 0.3a
 Asp 1.2 ± 0.1a
 Lys 34.8 ± 4.3a
 Arg 8.2 ± 2.5a
 GABA 8.3 ± 0.8a
Carbohydrates
 Suc 1.5 ± 0.6a
 Glc 1.2 ± 0.5a
Fru 1.5 ± 0.2a
 Mannitol 101.3 ± 10.3a
 Maltose 5.0 ± 1.0a
TCA cycle organic acids
 Citrate 1.1 ± 0.1a
 Aconitate 1.0 ± 0.1a
 Isocitrate 1.4 ± 0.2a
 α-Ketoglutarate 2.0 ± 0.3a
 Succinate 1.4 ± 0.1a
 Fumarate 1.0 ± 0.04a
 Malate 1.0 ± 0.01a
Glycolytic metabolites
 Gluconate 26.0 ± 3.2a
 Glycerol 13.3 ± 0.8a
 Glucose-6-phosphate 2.7 ± 0.2b
 Fructose-6-phosphate 5.3 ± 0.8b
 3-Phosphoglycerate 2.0 ± 0.1a
 PEP 8.0 ± 2.1c
 Pyruvate 3.1 ± 0.5c

Leaf disc samples were incubated for 4 h in the presence of the indicated exogenous metabolite, and the fold increase of that metabolite versus control incubations was determined. Significant increases are indicated in boldface (t test, P < 0.05).

a

Determined by gas chromatography-mass spectrometry.

b

Determined by liquid chromatography-quadrupole/time-of-flight-mass spectrometry.

c

Determined by enzymatic analysis.