Table 1. Accumulation of Exogenous Metabolites in Leaf Discs.
| Compound | Metabolite Level Ratio (Exposed/Nonexposed ± se) |
|---|---|
| Amino acids | |
| Gly | 5.2 ± 1.1a |
| Ala | 5.6 ± 0.2a |
| Val | 50.4 ± 5.1a |
| Leu | 105.6 ± 5.4a |
| Ile | 98.9 ± 9.1a |
| Met | 47.8 ± 4.3a |
| Phe | 39.9 ± 3.9a |
| Pro | 39.9 ± 4.2a |
| Ser | 15.0 ± 1.6a |
| Thr | 13.2 ± 1.0a |
| Asn | 6.0 ± 2.0a |
| Gln | 8.8 ± 0.9a |
| Glu | 1.4 ± 0.3a |
| Asp | 1.2 ± 0.1a |
| Lys | 34.8 ± 4.3a |
| Arg | 8.2 ± 2.5a |
| GABA | 8.3 ± 0.8a |
| Carbohydrates | |
| Suc | 1.5 ± 0.6a |
| Glc | 1.2 ± 0.5a |
| Fru | 1.5 ± 0.2a |
| Mannitol | 101.3 ± 10.3a |
| Maltose | 5.0 ± 1.0a |
| TCA cycle organic acids | |
| Citrate | 1.1 ± 0.1a |
| Aconitate | 1.0 ± 0.1a |
| Isocitrate | 1.4 ± 0.2a |
| α-Ketoglutarate | 2.0 ± 0.3a |
| Succinate | 1.4 ± 0.1a |
| Fumarate | 1.0 ± 0.04a |
| Malate | 1.0 ± 0.01a |
| Glycolytic metabolites | |
| Gluconate | 26.0 ± 3.2a |
| Glycerol | 13.3 ± 0.8a |
| Glucose-6-phosphate | 2.7 ± 0.2b |
| Fructose-6-phosphate | 5.3 ± 0.8b |
| 3-Phosphoglycerate | 2.0 ± 0.1a |
| PEP | 8.0 ± 2.1c |
| Pyruvate | 3.1 ± 0.5c |
Leaf disc samples were incubated for 4 h in the presence of the indicated exogenous metabolite, and the fold increase of that metabolite versus control incubations was determined. Significant increases are indicated in boldface (t test, P < 0.05).
Determined by gas chromatography-mass spectrometry.
Determined by liquid chromatography-quadrupole/time-of-flight-mass spectrometry.
Determined by enzymatic analysis.