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. 2020 Jan 6;32(3):630–649. doi: 10.1105/tpc.19.00569

Figure 3.

Figure 3.

The C-Terminal TMD Determines the Subcellular Localization of ONAC054 Proteins.

(A) Amino acid (aa) length of mature ONAC054 (ONAC054α), ONAC054△TM1(481-649), ONAC054△TM2(568-649), and ONAC054△TM3(627-649) proteins.

(B) Putative regions of the TMD in ONAC054 predicted by the Dense Alignment Surface (DAS) transmembrane prediction server (https://tmdas.bioinfo.se/DAS/index.html). aa, amino acid.

(C) Subcellular localization of GFP-ONAC054α, GFP-ONAC054△TM1, GFP-ONAC054△TM2, GFP-ONAC054△TM3, and GFP-ONAC054β examined by transient expression in onion epidermal cells. DIC, differential interference contrast. Scale bar, 10 μm.

(D) Gene structures of ONAC054α and ONAC054β. The insertion of 7 bp in the fifth intron of ONAC054β caused alternative 3′ splice site selection and a premature stop codon. Asterisks indicate the position of the premature stop codon. The blue box indicates the TM1 region of ONAC054α. aa, amino acid.

(E) Subcellular localization of GFP (control), GFP-ONAC054α, and GFP-ONAC054β examined by transient expression using protoplasts isolated from 10-d-old seedlings of rice. Scale bar, 10 μm. These experiments were repeated twice with similar results. Chl, chlorophyll.