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. 2020 Jan 17;32(3):740–757. doi: 10.1105/tpc.19.00685

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Figure 7. — PP95 Acts Antagonistically with CK2 to Modulate the ER Exit of PTs.

(A) PT8 protein levels at the PM and the ER in plants. Proteins were isolated from plants grown under Pi-sufficient conditions. Anti-PT8 antibody was used to detect PT8 protein levels. PHF1 and PIP1;3 were used as ER and PM protein controls, respectively.

(B) Subcellular localization of PT2-GFP and PT8-GFP fusion proteins in protoplasts. Pro35S:PT2/8-GFP (control), Pro35S:PT2-GFP, or Pro35S:PT8-GFP constructs together with Pro35S:CK2α3 (a3-OV) or Pro35S:CK2α3 and Pro35S:PP95 (a3-OV/PP95-OV) were transformed into rice protoplasts. Bars = 5 μm.

(C) Subcellular localization of PT8-GFP in root epidermal cells of 7-d-old ProPT8:PT8-GFP, ProPT8:PT8-GFP/a3-OV, and ProPT8:PT8-GFP/a3-OV/PP95-OV transgenic plants. Bars = 20 μm.