Figure 4.

HopO1-1 Promotes PD Permeability in Arabidopsis.

(A) Confocal images show the diffusion of YFP. Images were taken of leaf epidermal cells of Col-0 and transgenic plants expressing HopO1-1 variants. YFP and ER-CFP were cobombarded into the leaves of wild-type Col-0, 35S-HopO1-1, and 35S-HopO1-1^DD. PD-dependent diffusion of YFP molecules was examined. Asterisks indicate the bombarded sites expressing both YFP and ER-CFP. Bar = 50 µm.

(B) Quantitative data show the percentage of transformation events resulting in PD trafficking of YFP in wild-type Col-0, 35S-HopO1-1, and 35S-HopO1-1^DD. The degree of trafficking is scored by counting the number of transformation events yielding the diffusion of YFP to surrounding cells versus the total transformation events per experiment. Error bars represent se from three biological replicates. Statistical differences between wild-type Col-0 and the transgenic plants were analyzed with a two-tailed t test (**, P < 0.005).

(C) Quantitative data show the average number of cells containing YFP in wild-type Col-0, 35S-HopO1-1, and 35S-HopO1-1^DD. Transformation events resulting in PD trafficking from all three independent experiments are combined for the analysis. Error bars represent se. Statistical differences among wild-type Col-0 and the transgenic plants were analyzed with a Mann-Whitney U test (**, P < 0.0001). ND, no statistical difference.