FIG 3.

Lycopene production in E. coli strains harboring the archaeal MVA pathway. (A) The cell pellet of each strain cultivated aerobically. (B) The amount of lycopene extracted from each strain cultivated aerobically in the medium supplemented with or without 5.0 mg/ml MVL. Cultivation and lycopene extraction were performed in triplicate. Student’s t test values: **, P < 0.01; *, P < 0.03. (C) The cell pellet of each strain cultivated semianaerobically. (D) The amount of lycopene extracted from each strain cultivated semianaerobically in the medium supplemented with or without 5.0 mg/ml MVL. (E) The growth curves of dmMP7 and dNC strains with or without supplementation of MVL. Each strain was cultivated semianaerobically in a medium containing 100 μM fosmidomycin. Cultivation and measurement of OD₆₀₀ were performed in triplicate.