TABLE 1.
Plasmids and E. coli strains used in this study
| Plasmid or strain | Relevant characteristics | Reference or source |
|---|---|---|
| Plasmids | ||
| pBAD18 | AmpR, pBR322, PARA | 27 |
| pBAD-MVK | AmpR, pBR322, PARA, MM_1762 | This study |
| pBAD-mMP2 | AmpR, pBR322, PARA, JW2857, MM_1762 | This study |
| pBAD-mMP3 | AmpR, pBR322, PARA, MM_1763, JW2857, MM_1762 | This study |
| pBAD-mUA1 | AmpR, pBR322, PARA, MM_1524 | This study |
| pBAD-mUA2 | AmpR, pBR322, PARA, MM_1525, MM_1524 | This study |
| pBAD-mUA3 | AmpR, pBR322, PARA, MM_1871, MM_1525, MM_1524 | This study |
| pBAD-msUA3 | AmpR, pBR322, PARA, YDR538W, MM_1525, MM_1524 | This study |
| pBAD-mUA4 | AmpR, pBR322, PARA, MM_1526, MM_1871, MM_1525, MM_1524 | This study |
| pBAD-msUA4 | AmpR, pBR322, PARA, MM_1526, YDR538W, MM_1525, MM_1524 | This study |
| pBAD-mMP7 | AmpR, pBR322, PARA, MM_1526, MM_1871, MM_1525, MM_1524, MM_1763, JW2857, MM_1762 | This study |
| pBAD-msMP7 | AmpR, pBR322, PARA, MM_1526, YDR538W, MM_1525, MM_1524, MM_1763, JW2857, MM_1762 | This study |
| pBAD-aUA1 | AmpR, pBR322, PARA, APE_2089 | This study |
| pBAD-aUA2 | AmpR, pBR322, PARA, APE_2087.1, APE_2089 | This study |
| pBAD-aUA3 | AmpR, pBR322, PARA, APE_1647, APE_2087.1, APE_2089 | This study |
| pBAD-asUA3 | AmpR, pBR322, PARA, YDR538W, APE_2087.1, APE_2089 | This study |
| pBAD-aUA4 | AmpR, pBR322, PARA, APE_2078, APE_1647, APE_2087.1, APE_2089 | This study |
| pBAD-asUA4 | AmpR, pBR322, PARA, APE_2078, YDE538W, APE_2087.1, APE_2089 | This study |
| pBAD-aMP7 | AmpR, pBR322, PARA, APE_2078, APE_1647, APE_2087.1, APE_2089, MM_1763, JW2857, MM_1762 | This study |
| pBAD-asMP7 | AmpR, pBR322, PARA, APE_2078, YDE538W, APE_2087.1, APE_2089, MM_1763, JW2857, MM_1762 | This study |
| pBAD-mMPdelAMPD | AmpR, pBR322, PARA, MM_1871, MM_1525, MM_1524, MM_1763, JW2857, MM_1762 | This study |
| pBAD-mMPcomAMPD | AmpR, pBR322, PARA, MM_1871, MM_1525, MM_1524, MM_1763, JW2857, MM_1762, MM_1526 | This study |
| pBAD-mMPdelPMDh | AmpR, pBR322, PARA, MM_1526, MM_1871, MM_1763, JW2857, MM_1762 | This study |
| pBAD-mMPcomPMDh | AmpR, pBR322, PARA, MM_1526, MM_1871, MM_1763, JW2857, MM_1762, MM_1525, MM_1524 | This study |
| pACYC-IBE | TetR, p15A, CrtE, CrtI, CrtB | 15 |
| E. coli strain | ||
| TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG | Life Technologies, USA |
| ME9783 (SN1071) | F− thr-1 leuB6 thi-1 lacY1 galK2 ara-4 xyl-5 mtl-1 proA2 his-60 argE3 rpsL31 tsx-33 supE44 recB21 recC22 sbcA23 Δ(hsdR::frt) | NBRP-E. coli (NIG, Japan) (26) |
| DH1 | F− recA1 endA1 gyrA96 thi-1 hsdR17(r-K, m-K) supE44 relA1 λ- | NBRP-E. coli (NIG, Japan) |
| NC | TOP10 with pBAD18 and pACYC-IBE | This study |
| mMP7 | TOP10 with pBAD-mMP7 and pACYC-IBE | This study |
| msMP7 | TOP10 with pBAD-msMP7 and pACYC-IBE | This study |
| aMP7 | TOP10 with pBAD-aMP7 and pACYC-IBE | This study |
| asMP7 | TOP10 with pBAD-asMP7 and pACYC-IBE | This study |
| delAMPD | TOP10 with pBAD-mMPdelAMPD and pACYC-IBE | This study |
| comAMPD | TOP10 with pBAD-mMPcomAMPD and pACYC-IBE | This study |
| delPMDh | TOP10 with pBAD-mMPdelPMDh and pACYC-IBE | This study |
| comPMDh | TOP10 with pBAD-mMPcomPMDh and pACYC-IBE | This study |
| dNC | DH1 with pBAD18 | This study |
| dmMP7 | DH1 with pBAD-mMP7 | This study |