TABLE 2.

Primers used in this study

Gene	Primer sequence	Constructed plasmid
MM_1762	forward: 5′-AGCTGGGTACCAAGAGATATAATATGGTTTCATGTTCTGCGCC-3′	pBAD-MVK
	reverse: 5′-GATGCTCTAGATTAATCGACCTTCAACCCCTGTTC-3′
JW2857	forward: 5′-TTTTTTTGGGCTAGCGAATTCAGAGGAATTATAATGATAATGCAAACGGAACAC-3′	pBAD-mMP2
	reverse: 5′-CTTGGTACCGAGCTCGAATTATTATTTAAGCTGGGTAAATGCAG-3′
MM_1763	forward: 5′-TTTTTTTGGGCTAGCGAATTCAGGAGAAATATAATGAATGCTTCAAACGAACC-3′	pBAD-mMP3
	reverse: 5′-CATTATAATTCCTCTGAATTATTATACCCTTTTATCCGGACTG-3′
MM_1524	forward: 5′-TTTTTTTGGGCTAGCGAATTCAAGAGGAATATTATGATTCCCATTAAATTCAAAGG-3′	pBAD-mUA1
	reverse: 5′-CCGGGTACCGAGCTCGAATTATTATTTTTTTGCCTGCCCTTC-3′
MM_1525	forward: 5′-TTTTTTTGGGCTAGCGAATTCAAGGAGAATTATATGTATCTAACAAAAGAAGAAGAACAAATC-3′	pBAD-mUA2
	reverse: 5′-CATAATATTCCTCTTGAATTATTAATGGGAATCACCTGCC-3′
MM_1871	forward: 5′-TTTTTTTGGGCTAGCGAATTCAGGAGGAATAATATGGAAATAATTGTAGGCATCAG-3′	pBAD-mUA3
	reverse: 5′-CATATAATTCTCCTTGAATTATTATCCGTTGTTCTCCAAATC-3′
YDR538W	forward: 5′-TTTTTTTGGGCTAGCGAATTCAGGAGGAATAATATGCTCCTATTTCCAAGAAGAAC-3′	pBAD-msUA3
	reverse: 5′-CATATAATTCTCCTTGAATTATTACTTGCTTTTTATTCCTTCCC-3′	pBAD-asUA3
MM_1526	forward: 5′-TTTTTTTGGGCTAGCGAATTCAAGAAGATTATTATGACTTATAGAGATTTTATAGGCCG-3′	pBAD-mUA4
	reverse: 5′-CATATTATTCCTCCTGAATTATTATTCTTCCGGAATTATTGC-3′	pBAD-msUA4
MM_1526–MM_1524	forward: 5′-TTTTTTTGGGCTAGCGAATTCAAGAAGATTATTATGACTTATAGAGATTTTATAGGCCG-3′	pBAD-mMP7
	reverse: 5′-CATTATATTTCTCCTGAATTATTATTTTTTTGCCTGCCCTTC-3′	pBAD-msMP7
APE_2089	forward: 5′-TTTTTTTGGGCTAGCGAATTCAAGAGGAATATTATGGAGGTCCTCAGGTACAG-3′	pBAD-aUA1
	reverse: 5′-CCGGGTACCGAGCTCGAATTATTAGGGCTGGCCAGG-3′
APE_2087.1	forward: 5′-TTTTTTTGGGCTAGCGAATTCAAGGAGAATTATATGTACCTGACGAGGGAGG-3′	pBAD-aUA2
	reverse: 5′-CATAATATTCCTCTTGAATTTTACGACACCAGCTTTGGC-3′
APE_1647	forward: 5′-TTTTTTTGGGCTAGCGAATTCAGGAGGAATAATATGTCCTGCAGACCTAGCTCGG-3′	pBAD-aUA3
	reverse: 5′-CATATAATTCTCCTTGAATTATTAATCCCCCTCCTCGG-3′
APE_2078	forward: 5′-TTTTTTTGGGCTAGCGAATTCAAGAAGATTATTATGGAGGATGCGAGTCTAAAG-3′	pBAD-aUA4
	reverse: 5′-CATATTATTCCTCCTGAATTATTAGTCTCCCGGCTGGTG-3′	pBAD-asUA4
APE_2078–APE_2089	forward: 5′-TTTTTTTGGGCTAGCGAATTCAAGAAGATTATTATGGAGGATGCGAGTCTAAAG-3′	pBAD-aMP7
	reverse: 5′-CATTATATTTCTCCTGAATTATTAGGGCTGGCCAGG-3′	pBAD-asMP7
MM_1871/MM_1525/MM_1524	forward: 5′-TTTTTTTGGGCTAGCGAATTCAGGAGGAATAATATGGAAATAATTGTAGGCATCAG-3′	pBAD-mMPdelAMPD
	reverse: 5′-CATTATATTTCTCCTGAATTATTATTTTTTTGCCTGCCCTTC-3′
MM_1526	forward: 5′-GGAACAGGGGTTGAAGGTCGATTAATCTAGCAAGAAGATTATTATGACTTATAGAGATTTTATAGG-3′	pBAD-mMPcomAMPD
	reverse: 5′-CAAGCTTGCATGCCTGCAGGTCGACTCTAGATTATTCTTCCGGAATTATTGC-3′
MM_1526/MM_1871	forward: 5′-TCCATACCCGTTTTTTTGGGCTAGCGAATTCAAGAAGATTATTATGACTTATAGAGATTTTATAGGCCG-3′	pBAD-mMPdelPMDh
	reverse: 5′-TTGAAGCATTCATTATATTTCTCCTGAATTATTATCCGTTGTTCTCCAAATC-3′
MM_1525/MM_1524	forward: 5′-GGAACAGGGGTTGAAGGTCGATTAATCTAGCAAGGAGAATTATATGTATCTAACAAAAGAAGAAG-3′	pBAD-mMPcomPMDh
	reverse: 5′-CAAGCTTGCATGCCTGCAGGTCGACTCTAGATTATTTTTTTGCCTGCCCTTC-3′