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Plasmid maps for pPUZZLE_mPOS5 and pPUZZLE_cPOS5. In red, the restriction enzymes used were SbfI and SfiI for cloning mPOS5 and cPOS5, AscI for plasmid linearization, and BglII for plasmid ligation verification. pPUZZLE contains the kanMX gene encoding kanamycin resistance (E. coli) and Geneticin G418 resistance (P. pastoris). ORI, origin of replication.
