TABLE 3.
Primers used in this study
| Primer | DNA sequence (5′ to 3′)a | Description |
|---|---|---|
| aceAB-F | TAAGAAGGAGATATACATATGTCGTGCCGATGCGCACGC | Forward primer to amplify aceAB with an NdeI site |
| aceAB-R | GTGGTGGTGGTGGTGCTCGAGGCCGATGATCTCGTCCATGG | Reverse primer to amplify aceAB with an XhoI site |
| RT-16SF | ATCGTTTAGGGCGTGGACT | Forward primer for quantitative real-time PCR to amplify a 128-bp fragment of 16S rRNA |
| RT-16SR | GAAATGCGTAGATATGTGGAGG | Reverse primer for quantitative real-time PCR to amplify a 128-bp fragment of 16S rRNA |
| RT-aceAF | CCGACTGGCTGGACTACTTCTA | Forward primer for quantitative real-time PCR to amplify a 105-bp fragment of aceA |
| RT-aceAR | CCTTGCTCTGATCGCTGTTT | Reverse primer for quantitative real-time PCR to amplify a 105-bp fragment of aceA |
| RT-aceBF | ACGCAGGTGATGCAGAATG | Forward primer for quantitative real-time PCR to amplify a 120-bp fragment of aceB |
| RT-aceBR | GTCGTAGGTGGATAGACCGTAG | Reverse primer for quantitative real-time PCR to amplify a 120-bp fragment of aceB |
| aceA-F | TAAGAAGGAGATATACATATGATCACTTCTCCATAC | Forward primer to amplify aceA with an NdeI site |
| aceA-R | GTGGTGGTGGTGGTGCTCGAGGACGGCCACGCCGATCTTC | Reverse primer to amplify aceA with an Xhol site |
| aceB-F | TAAGAAGGAGATATACATATGAACTATATTCCGGTCAAGG | Forward primer to amplify aceB with an NdeI site |
| aceB-R | GTGGTGGTGGTGGTGCTCGAGGCCGATGATCTCGTCCATGG | Reverse primer to amplify aceB with an Xhol site |
| aceB-DF | TATGACCATGATTACGAATTCAGGGCGCTCAGCCGAGGC | Forward primer to amplify aceB for gene deletion |
| aceB-DR | CAGGTCGACTCTAGAGGATCCAGAGCAGATCTTCCCCCTCG | Reverse primer to amplify aceB for gene deletion |
| aceB-CF | GATAAGCTTGATATCGAATTCGGCCTCTCCCGGATTCTCT | Forward primer to amplify aceB with an EcoRI site for gene complementation |
| aceB-CR | CGCTCTAGAACTAGTGGATCCTCAGCCGATGATCTCGTCCA | Reverse primer to amplify aceB with a BamHI site for gene complementation |
a
Underlined sequences are restriction enzyme cutting sites.