TABLE 3.
No. | Taxon | Strain | ANIb
(lower diagonal) or dDDH (upper diagonal) (%) |
||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | |||
1 | C. michiganensis sensu stricto | LMG 7333T | 57.70 | 58.70 | 48.00 | 48.60 | 49.70 | 48.00 | 49.80 | 49.50 | 44.80 | 43.10 | 40.20 | 40.00 | 37.50 | 37.20 | 36.80 | 34.60 | |
2 | C. michiganensis subsp. californiensis | CFBP 8216T | 95/95/95 | 57.80 | 46.40 | 47.90 | 48.60 | 47.40 | 48.10 | 47.90 | 44.80 | 43.70 | 40.50 | 40.40 | 38.50 | 38.40 | 38.00 | 35.70 | |
3 | Clavibacter sp. | LMG 26808 | 95/94/95 | 94/95/95 | 45.80 | 47.80 | 48.40 | 47.10 | 47.90 | 47.70 | 44.30 | 42.60 | 39.60 | 39.50 | 37.10 | 37.00 | 36.40 | 34.50 | |
4 | C. sepedonicus | ATCC 33113T | 92/92/92 | 92/92/92 | 92/92/92 | 45.10 | 46.20 | 45.20 | 47.00 | 46.60 | 43.90 | 42.30 | 39.10 | 39.10 | 36.40 | 36.20 | 36.00 | 34.00 | |
5 | C. insidiosus | LMG 3663T | 93/93/93 | 93/93/93 | 92/92/93 | 92/92/92 | 64.60 | 59.90 | 51.20 | 51.00 | 47.10 | 43.30 | 40.50 | 40.20 | 37.90 | 37.40 | 37.20 | 34.80 | |
6 | Clavibacter sp. | CFBP 7494 | 93/93/93 | 92/93/93 | 93/92/93 | 92/92/92 | 96/96/96 | 60.20 | 52.20 | 52.10 | 46.00 | 44.10 | 40.70 | 40.60 | 38.10 | 37.60 | 37.50 | 34.90 | |
7 | C. nebraskensis | NCPPB 2581T | 92/93/92 | 92/93/92 | 92/92/92 | 92/92/92 | 95/95/95 | 95/95/95 | 51.40 | 51.10 | 45.30 | 43.30 | 40.70 | 40.70 | 37.70 | 37.20 | 36.90 | 34.90 | |
8 | C. michiganensis subsp. chilensis | CFBP 8217T | 93/93/93 | 93/93/93 | 92/93/93 | 92/93/92 | 93/93/93 | 93/93/94 | 93/93/93 | 87.50 | 47.00 | 45.80 | 43.40 | 42.90 | 40.20 | 39.50 | 39.20 | 37.10 | |
9 | C. michiganensis subsp. phaseoli | CFBP 8627T | 93/93/93 | 93/93/93 | 92/92/93 | 92/93/92 | 93/93/93 | 93/93/93 | 93/93/93 | 99/99/99 | 47.10 | 45.70 | 43.30 | 42.90 | 40.00 | 39.50 | 39.10 | 37.00 | |
10 | Clavibacter sp. | CFBP 8615 | 92/92/92 | 92/92/92 | 91/92/92 | 91/92/92 | 92/92/92 | 92/92/92 | 92/92/92 | 92/92/92 | 92/93/92 | 48.00 | 40.90 | 40.50 | 38.20 | 37.60 | 37.70 | 35.80 | |
11 | Clavibacter sp. | CFBP 8019 | 91/92/91 | 91/92/91 | 91/91/91 | 91/92/91 | 91/92/91 | 91/91/91 | 91/91/91 | 92/92/92 | 92/92/92 | 93/93/93 | 40.10 | 40.00 | 36.90 | 36.80 | 36.80 | 34.40 | |
12 | C. capsici | PF008T | 90/91/90 | 90/91/91 | 90/90/90 | 90/91/90 | 90/91/90 | 90/90/90 | 90/91/90 | 91/92/91 | 91/92/91 | 90/91/91 | 90/91/90 | 58.50 | 38.40 | 38.50 | 37.70 | 34.50 | |
13 | Clavibacter sp. | CF11 | 90/90/90 | 90/90/90 | 90/90/90 | 90/90/90 | 90/90/90 | 91/90/90 | 91/90/90 | 91/91/91 | 91/91/91 | 90/90/90 | 90/90/90 | 95/95/95 | 38.10 | 38.50 | 37.60 | 34.40 | |
14 | C. tessellarius | ATCC 33566T | 90/90/89 | 90/91/90 | 89/89/89 | 89/90/89 | 90/90/89 | 90/90/90 | 90/90/89 | 91/91/90 | 91/91/90 | 90/90/90 | 89/90/89 | 90/91/90 | 90/90/90 | 49.00 | 57.70 | 33.50 | |
15 | Clavibacter sp. | DOAB 609 | 89/89/89 | 89/90/90 | 89/89/89 | 89/89/89 | 89/89/89 | 90/90/89 | 89/89/89 | 90/90/90 | 90/90/90 | 89/90/89 | 89/89/89 | 90/90/90 | 90/90/90 | 93/93/93 | 47.20 | 33.60 | |
16 | Clavibacter sp. | CFBP 8017 | 89/89/89 | 89/90/90 | 89/89/89 | 89/89/89 | 89/89/89 | 90/90/89 | 89/89/89 | 90/90/90 | 90/90/90 | 89/90/89 | 89/89/89 | 90/90/89 | 90/90/89 | 95/95/95 | 92/92/92 | 33.30 | |
17 | Clavibacter sp. | CASJ009 | 88/89/89 | 89/90/88 | 88/88/88 | 88/89/88 | 88/89/88 | 88/88/88 | 88/89/88 | 89/90/89 | 89/90/90 | 89/90/89 | 88/89/88 | 88/89/89 | 88/88/88 | 88/89/88 | 88/88/87 | 87/88/87 |
A combination of ANI and dDDH indices was used to designate a taxonomic status for a given phylogenetic clade, where the “new species” status was assigned to a clade only when both the ANI and dDDH values were below the accepted thresholds (≤95% and ≤70% for ANI and dDDH, respectively [21]).
ANI values were calculated using three different algorithms, i.e., JSpeciesWS, ANI calculator, and OrthoANIu, which are presented from left to right, respectively.