TABLE 1.
Culture-based detection of STEC and precipitation data reported in inches
| Sample IDa | Date collected (mo/day/yr) | STECb | No. of copies stx2/μg DNAc | Precipitation (in)d
|
|
|---|---|---|---|---|---|
| Day 1 | 5-day sum | ||||
| GABOSR | |||||
| G130904 | 9/4/13 | − | 8.1 | 0 | 0 |
| G140116 | 1/16/14 | + | 8 | 0 | 0.01 |
| G140128 | 1/28/14 | + | 0 | 0 | 0 |
| G140210 | 2/10/14 | + | 4.4 | 0.01 | 1.1 |
| G140224 | 2/24/14 | + | 1.8 | 0 | 0 |
| G140301 | 3/1/14 | + | 1.5 | 0.33 | 2.01 |
| G140319 | 3/19/14 | − | 0 | 0 | 0.01 |
| G140402 | 4/2/14 | + | 1.4 | 0.03 | 1.04 |
| G140415 | 4/15/14 | − | 0 | 0 | 0 |
| G140611 | 6/11/14 | − | 2.4 | 0 | 0 |
| TOWOSR | |||||
| T130904 | 9/4/13 | − | 14.2 | 0 | 0 |
| T130918 | 9/18/13 | + | 15.3 | 0 | 0 |
| T131023 | 10/23/13 | − | 0 | 0 | 0 |
| T131230 | 12/30/13 | + | 3.9 | 0 | 0 |
| T140116 | 1/16/14 | − | 0 | 0 | 0.01 |
| T140128 | 1/28/14 | + | 0 | 0 | 0 |
| T140210 | 2/10/14 | − | 1.7 | 0.01 | 1.1 |
| T140224 | 2/24/14 | − | 1.5 | 0 | 0 |
| T140319 | 3/19/14 | − | 0 | 0 | 0.01 |
| T140611 | 6/11/14 | − | 0 | 0 | 0 |
| Upstream GABOSR control | |||||
| GC1 | 3/9/16 | − | 0 | 0 | 2.84 |
| GC2 | 3/9/16 | + | 0 | 0 | 2.84 |
| GC3 | 3/9/16 | − | 0 | 0 | 2.84 |
| Upstream TOWOSR control | |||||
| TC1 | 4/19/17 | + | 0 | 0 | 0.45 |
| TC2 | 4/19/17 | − | 0 | 0 | 0.45 |
| West Salinas | |||||
| WS1 | 5/4/17 | − | 0 | 0 | 0 |
| WS2 | 5/4/17 | − | 0 | 0 | 0 |
a
ID, identifier; GABOSR, Gabilan at Old Stage Road; TOWOSR, Towne Creek at Old Stage Road.
b
Samples in which STEC was detected by PCR of enrichment cultures are listed as either positive (+) or negative (−).
c
Copy number of the Shiga toxin gene (stx2) was determined via ddPCR.
d
Precipitation levels (in inches) for the day of sample collection (day 1) and the sum of precipitation levels for five days prior to the sampling day (5-day sum) were obtained from the California Irrigation Management Information System database (http://ipm.ucanr.edu/WEATHER/) for North Salinas weather station (the closest monitoring station to the downstream sites).