TABLE 3.

Summary of the findings of the three candidate genes

Gene	Observations	Suggested reannotation
Pseudomonas sp. 9.1 FEZ21_09870	Clear reductase activity with vanillin and coniferyl aldehyde; no oxidizing activity with aromatic alcohols detected; confirmation of in silico predicted activity	Psp91_areA (vanillin reductase/aromatic aldehyde reductase gene)
P. putida KT2440 PP_2426 (calA)	High sequence identity to FEZ21_09870 (82.3%; BLASTp); identical activity to FEZ21_09870; unexpected reductase activity on coniferyl aldehyde, instead of the proposed oxidative activity (39); reannotation needed	areA (vanillin reductase/aromatic aldehyde reductase gene)
P. putida KT2440 PP_3839 (adhP)	No activity with aromatic aldehydes; rapid conversion of coniferyl alcohol into coniferyl aldehyde; likely the true P. putida coniferyl alcohol dehydrogenase, a function previously incorrectly attributed to PP_2426	calA-II (coniferyl alcohol dehydrogenase gene)