TABLE 1.
Primers used in this study
| Primer | Sequence, 5′→3′a | Purpose |
|---|---|---|
| Fu/Cu | GGAAATTAAGCTATGCACCGCAGTATAGTC | crgA cloning/complementation |
| Fd/Cr | TATTTTCATATGGAACAAGATTTGTCTATA | |
| Cd | CGTGGATCCATTGTCGAACGACAAGGCAGT | |
| Cf | TCAAAAATATTAAATGCTAAAAATGGAGAA | |
| hF | GTCGGAGACAGAAGATGATATTGAAGGAG | hph cloning |
| hR | GTTGGAGATTTCAGTAACGTTAAGTGGAT | |
| 5f | GGAAATTAAGCTATGCACCGCAGTATAGTCA | Cloning of 5′ flank region of crgA |
| 5r | ATCCACTTAACGTTACTGAAATCTCCAACAGFGAAGGTTTGAACAGAAAACTCTTGTAGC | |
| 3f | CTCCTTCAATATCATCTTCTGTCTCCGACACAGACGACTGAAGAGATGATTGATGAACT | Cloning of 3′ flank region of crgA |
| 3r | TATTTTCATATGGAACAAGATTTGTCTATACTG | |
| hf | GCGAAGAATCTCGTGCTTTCA | Cloning of split marker |
| hr | TCCAGAAGAAGATGTTGGCGAC | |
| P1 | AGCCTACGTTTTGAGTAGCTCGATC | Confirming mutants |
| P2 | ATACATTGTTGTGATGAAGCCACAC | |
| P3 | ATGGGCATGTTTTTGGGCTAGCAGT | |
| P4 | CGCGCAGGCTCTCGATGAGCTGAT | |
| P5 | CTCCTACATCGAAGCTGAAAGCACG | |
| P6 | ACTCCTCTTCCAAGAGCACTAGGTA | |
| hmgR-F | AAACGATGGATTGAACAAGAGGG | RT-qPCR |
| hmgR-R | TAGACTAGACGACCGGCAAGAGC | |
| carB-F | TATTGGCGGAACTGCTACTGC | |
| carB-R | CCCTGATCAAAGCGATGACC | |
| carRA-F | TCTTGAGCGTCGTCCTATCC | |
| carRA-R | GCACGGTCAATTATCCAAGC | |
| tef1-F | AACTCGGTAAGGGTTCCTTCAAG | |
| tef1-R | CGGGAGCATCAATAACGGTAAC |
a
Sequences in boldface are the reverse complement of hR and hF, respectively. The hph was inserted in the direction of crgA antisense.