Table 1.
Data collection and refinement statistics.
| Ube2D1 + Ubv.D1.1 | Ube2V1 + Ubv.V1.1 + Ube2N | Ube2G1 + Ubv.G1.1 | |
|---|---|---|---|
| Data collection | |||
| Wavelength | 0.9792 | 0.9792 | 0.9795 |
| Space group | P212121 | P32 | P3221 |
| Cell dimensions a, b, c (Å) | 51.12, 52.56, 96.75 | 91.44, 91.44, 92.45 | 66.54, 66.54, 225.1 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 120 | 90, 90, 120 |
| Resolution, Å | 50–2.10 | 40–2.55 | 46–2.35 |
| Rmerge, % | 10.4 (88.1) | 6.5 (95.0) | 6.0 (36.7) |
| CC1/2 | 0.999 (0.610) | 0.993(0.572) | 0.967 (0.913) |
| I/σ | 30.2 (1.0) | 26.7 (1.1) | 23.3 (2.8) |
| Completeness, % | 99.9 (100) | 99.8 (99.9) | 99.6 (98.9) |
| Reflections total/unique | 112423/15572 | 142899/28109 | 168582/24851 |
| Redundancy | 7.2 (6.7) | 5.1 (4.9) | 6.8 (6.2) |
| Refinement | |||
| Rwork/Rfree | 0.214/0.231 | 0.271/0.287 | 0.206/0.257 |
| No. of atoms | |||
| Protein | 3402 | 9966 | 7428 |
| Water | 24 | 0 | 45 |
| B-factors, Å2 | |||
| Protein | 51.8 | 104.4 | 70.2 |
| water | 39.8 | – | 61.5 |
| R.M.S deviations | |||
| Bond lengths, Å | 0.002 | 0.004 | 0.002 |
| Bond angles, ° | 0.60 | 0.63 | 0.58 |
| Ramachandran plot | |||
| Most favored regions (%) | 99.1 | 98.1 | 99.1 |
| Allowed regions (%) | 0.9 | 1.9 | 0.9 |
| Disallowed regions (%) | 0.0 | 0.0 | 0.0 |
| MolProbity Score | 1.09 | 1.13 | 0.84 |
| PDB ID code | 6D4P | 6D6I | 6D68 |
Values in parentheses are for the highest resolution shell.