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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Shock. 2020 Aug;54(2):245–255. doi: 10.1097/SHK.0000000000001445

Figure 1. MKT-077 protects against LPS-induced lung inflammation and injury.

Figure 1.

Figure 1.

Wild type C57BL/6L mice were injected i.p. with MKT-077 (5 mg/kg) 1 h prior to or 1 h after aerosolizing the mice with E. coli LPS (0.5mg/ml; 6ml) for 30 min. Mice injected i.p. with saline 1 h prior to aerosolization served as control. After 16 h, lung homogenates were analyzed for (A) MPO activity, (C) IL-1β, (D) TNFα, (E) E-selectin and BAL fluid was analyzed for (B) albumin. Wild type C57BL/6L mice were injected i.p. with MKT-077 (5 mg/kg) 1 h prior to aerosolizing the mice with E. coli LPS (0.5mg/ml; 6ml) for 30 min. Six, sixteen and twenty four hours post LPS challenge, PaO2 was measured using MouseOx™ Pulse-oximeter as described in Methods (F&G). Wild type C57BL/6L mice were injected i.p. with MKT-077 (5 mg/kg) 1 h prior to aerosolizing the mice with E. coli LPS (0.5mg/ml; 6ml) for 30 min. After 16 h, lung homogenates were analyzed for levels of mortalin (H). Actin was used as a loading control.