Figure 6. Mortalin regulates thrombin-induced Ca²⁺ release and entry in EC.

(A&B) HPAEC grown to confluence on 25 mm coverslips were left untreated or treated with 1 µg/ml of MKT-077 for 1 h. Cells were then loaded with Fura2-AM for 30 min and washed twice with Ca²⁺ free HBSS buffer and mounted on an inverted microscope. Calcium release from the intracellular stores was determined by perfusing Ca²⁺ free imaging buffer and stimulating cells with thrombin (2.5 U/ml). Store-operated Ca²⁺ entry was measured following addition of 1.26 mM Ca²⁺. Fura-2 ratio (F-ratio 340/380) was calculated and analyzed with NIS-Element AR 3.0 Software.