Table 2.

Lower limit of detection (LLOD) with MRM-IDA-EPI scan and lower limit of quantitation (LLOQ) with MRM scan, and calibration profile of 18 chemotherapy agents in plasma.

Analyte	LLODa (ng/mL)	LLOQa (ng/mL)	Slope	Intercept	R2	Linear range (ng/mL)
1. Gemcitabine (GCA)	1.00	0.50	0.00927	7.8445E-04	0.99523	0.50–50
2. Dacarbazine (DACA)	0.50	0.50	0.01128	0.00263	0.99767	0.50–50
3. Fludarabine (FLD)	2.50	0.10	0.03272	0.00122	0.99266	0.10–50
4. Methotrexate (MTX)	0.25	0.10	0.28728	0.01918	0.99535	0.10–50
5. Topotecan (TOP)	0.10	0.10	0.03797	1.3332E-05	0.99619	0.10–50
6. Mitomycin (MIT)	0.25	0.10	0.05579	7.9954E-05	0.99853	0.10–50
7. Pemetrexed (PTR)	0.25	0.10	0.03728	−1.9923E-04	0.99745	0.10–50
8. Irinotecan (IRI)	0.50	0.10	0.02392	3.8156E-04	0.99327	0.10–50
9. Vincristine (VCR)	0.25	0.10	0.03453	−9.0884E-05	0.99642	0.10–50
10. Doxorubicin (DOXO)	0.25	0.10	0.01980	0.00249	0.99705	0.10–50
11. Vinblastine (VBL)	0.50	0.10	0.04683	−5.6620E-04	0.99543	0.10–50
12. Vinorelbine (VIN)	1.00	0.10	0.06812	−0.00168	0.99748	0.10–50
13. Bendamustine (BEN)	0.10	0.10	0.19672	3.7972E-04	0.99671	0.10–50
14. Ifosfamide (IF)	0.10	0.10	0.12948	−3.8529E-04	0.99693	0.10–50
15. Cyclophosphamide (CP)	0.25	0.10	0.08087	0.00123	0.99613	0.10–50
16. Etoposide (ETOP)	0.25	0.10	0.04023	1.7948E-04	0.99783	0.10–50
17. Docetaxel (DOCE)	0.5	1.00	4.15799E-04	2.37008E-04	0.99673	1.00–50
18. Paclitaxel (TAX)	0.5	1.00	0.00209	0.00195	0.99665	1.00–50

^aThe LLOD and LLOQ were derived using two different methods (see Table 2 caption). LLOQ is often equivalent or a higher concentration than LLOD; however, LLOD relied on IDA while LLOQ did not and so was less sensitive in this scenario.