Table 3.

Long term treatments with ELQ-121, ELQ-136 and ELQ-334 act parasitostatic.

Compound	Treatment culture time (days)	Post-treatment culture until host cell lysis
ELQ 121	3	17
	6	23
	9	23
	13	21
	20	18
	23	18
ELQ 136	3	16
	6	14
	9	11
	13	14
	20	13
	23	10
ELQ 334	3	10
	6	11
	9	11
	13	10
	20	ND
	23	ND

T25 tissue culture flasks were infected with B. besnoiti tachyzoites, and the compounds were added all at 2.5 μM concentration. Culture continued for the time spans indicated, with medium changes and addition of fresh compounds every 3–4 days. The cultures were then washed with medium to remove the drugs and then incubated further in the absence of the compounds until host cell lysis occurred. ND, not done.