Table 5.

Effect of GSM diet on microbiota of DSS-treated piglets (filum).

	Control	DSS	GSM	DSS+GSM	DSS effect			GSM effect			DSS+GSM effect
					η2	P-value	ω2	η2	P-value	ω2	η2	P-value	ω2
Firmicutes (r.a)	0.60 ± 0.01	0.54 ± 0.01	0.60 ± 0.01	0.50 ± 0.01	0.607	<0.0001*	0.578	0.053	0.1026	0.034	0.015	0.373	−0.002
Bacteroidetes (r.a)	0.36 ± 0.01	0.38 ± 0.01	0.36 ± 0.01	0.45 ± 0.01	0.375	0.0005*	0.347	0.112	0.0332*	0.089	0.133	0.0217*	0.109
Proteobacteria (r.a.)	0.01 ± 0.00	0.03 ± 0.00	0.03 ± 0.00	0.02 ± 0.00	0.094	0.0032*	0.085	0.065	0.0108	0.057	0.693	<0.0001*	0.679
Lactobacillus (r.a.)	0.30 ± 0.02	0.12 ± 0.01	0.09 ± 0.01	0.03 ± 0.00	0.285	<0.0001*	0.277	0.535	<0.0001*	0.526	0.074	0.0023*	0.068
Prevotella (r.a.)	0.23 ± 0.01	0.21 ± 0.02	0.22 ± 0.02	0.33 ± 0.00	0.118	0.0378*	0.093	0.197	0.0096*	0.17	0.258	0.0039*	0.23
Megasphaera (r.a.)	0.0003 ± 0.00	0.068 ± 0.00	0.222 ± 0.00	0.211 ± 0.02	0.021	0.0414*	0.016	0.864	<0.0001*	0.856	0.038	0.0076*	0.034
CF231 (r.a.)	0.016 ± 0.00	0.034 ± 0.00	0.01 ± 0.00	0.007 ± 0.0	0.115	0.0008*	0.107	0.543	<0.0001*	0.532	0.215	<0.0001*	0.207
Anaerovibrio (r.a.)	0.004 ± 0.00	0.0073 ± 0.00	0.03 ± 0.00	0.027 ± 0.0	0.00002	0.9769	−0.02	0.553	0.0002*	0.516	0.007	0.6025	−0.017
Roseburia (r.a)	0.015 ± 0.00	0.027 ± 0.00	0.005 ± 0.00	0.013 ± 0.00	0.339	<0.0001*	0.328	0.49	<0.0001*	0.477	0.004	0.2529	0.004

r.a., relative abundance; η², Eta squared; ω², Omega squared; Microbiota (filum) relative abundance values are represented as mean with their standard errors. ANOVA (two-way) was performed to analyse the main factors DSS, GSM, and DSS × GSM interaction effects on microbiota incidence. Calculation and effect size magnitudes are given in Materials and Methods section.

^*P-values lower than 0.05 are statistically significant.