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Overview of the keratinocyte polymicrobial colonization model. (A) The steps required to establish polymicrobial colonization of keratinocytes. (B) 3D projection of a dual species biofilm (red: P. aeruginosa-mCherry, green: S. aureus-GFP) set up as described in panel A. (C) Visualization of the HaCat monolayer beneath the dual species polymicrobial biofilm shown in panel B. Keratinocytes were stained with CellTracker and imaged with 63 × magnification. Scale bar indicates 20 μm.
