FIGURE 4.

Commensals reduce biofilm biomass for both S. aureus and P. aeruginosa individually and when co-inoculated. The HaCat monolayer was inoculated with the indicated bacterial species and incubated for 40 h (S. aureus, commensals) or 20 h (P. aeruginosa) as outlined in Figure 1. The biofilm biomass was calculated using ImageJ. SE: S. epidermidis; ML: M. luteus; SA: S. aureus; PA: P. aeruginosa. Bold font indicates the bacterial species quantified. ^∗p-value < 0.05. Images of the biofilms and HaCat monolayer are shown in Supplementary Figure S1, and average thickness and surface area are shown in Supplementary Figure S2.