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. 2020 Mar 3;10:3950. doi: 10.1038/s41598-020-60862-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Taxonomic composition of rat gut microbiota at the phylum level determined by 16S rRNA gene sequencing analysis. (B) The chao1 estimates. (C) Phylogenetic diversity (PD) estimates. (D) Total observed operational taxonomic units (OTUs). Alpha diversity analysis for rat gut microbiota from adulthood stressed group (L-Str), adulthood non-stressed group (L-Non), adolescent stressed group (S-Str), and adolescent non-stressed group (S-Non) were compared. € Beta diversity analysis by Principal coordinate analysis using an unweighted UniFrac metric.