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. 2020 Mar 3;10:3906. doi: 10.1038/s41598-020-60935-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chromatin immunoprecipitation analysis. (a) shows the nucleotide sequence between the physical location of 59122186 to 59122473 nucleotides on chromosome 10 in the bovine genome. Manually curated TATA box and the putative HIF1 binding sites were highlighted in blue and yellow colors. The primer binding sites were indicated with an underline. (b) adopted JASPAR Sequence logo of the HIF1 binding site based on ChIP seq data in humans. (c) indicates the agarose gel pictures of immune-precipitated and input DNA samples. (d,e) depict the relative quantification of HIF1 binding events on the CYP19A1 promoter based on percent input and fold change methods, respectively under FSH and IGF1 Supplemented conditions. Data were represented in mean ± SEM values of three independent experiments (n = 3). Significant differences were acknowledged at the minimum level of p < 0.05 by one way repeated measures analysis of variance. Pairwise comparisons were analyzed using Post hoc Tuckey test.