Figure 3.

Expansion of ovarian cancer TILs with anti-CTLA-4. TIL cultures were established from tumor fragments and subsequently rapidly expanded over a course of 14 days. For each patient, two initial and four expanded TIL cultures were established. Both the initial TIL culture and the rapid expansion were performed with high-dose IL-2 alone or supplemented with anti-CTLA-4 antibody. (A) Bar charts illustrating the TIL yield per fragment of the initial TIL cultures for each patient. (B) Scatter plot showing the TIL yield per fragment of the initial TIL culture under the two culture conditions. The dotted line indicates the threshold for a successful TIL culture. Data are presented with median. (C) Scatter plots showing the fold expansion during the rapid expansion with high dose IL-2 alone (upper figure) or additionally supplemented with anti-CTLA-4 antibody. The legend indicates the culture conditions during the initial TIL expansion. Data are presented with median. The culture conditions were compared using Wilcoxon signed rank test. Statistically significant differences are indicated with *, ** or *** for p-values less than 0.05, 0.01 or 0.001, respectively.