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. 2020 Mar 3;10:3951. doi: 10.1038/s41598-020-59043-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ethanol induced gene expression changes was reversed by sox2 mRNA injection. (A–D) Whole mount ISH shows reduced dld expression after ethanol exposure, which was like control in sox2 mRNA injected and sox2 mRNA injected plus ethanol-treated embryos. (E) Quantitative PCR showed downregulation of sox2, her7, and dlc transcripts in the ethanol-treated embryos, which increased in the sox2 mRNA injected plus ethanol-treated embryos.