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. 2020 Mar 3;10:3939. doi: 10.1038/s41598-020-60466-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-course transcriptome analysis of differentiation during early and late passages. Comparison of expression profiles between early (red bars) and late (blue bars) passages at three time points [human induced pluripotent stem cells (hiPSCs), the middle stage of differentiation, and the final stage of differentiation]. Forty similar expression patterns (SEPs) and five differential expression profiles (DEPs) are shown in the top and bottom panels, respectively. The normalized expression values were applied to calculate relative expression. The number at the top of each graph indicates the number of clusters. ODEP: obscure DEP.