Fig. 3. siPD-L1 induced a reduction of p-Akt and EGFR expression in the JHU006 chemoresistant cell line but not in chemosensitive JHU020 cells.

a The expression of Akt, p-Akt, p-P53 and EGFR 48 h after the application of siPD-L1. b The intensities of the protein bands illustrated in a were quantified using the ImageJ program. b demonstrates that the addition of siRNA-PD-L1 to the cells resulted in statistically significant decreases in Akt, p-Akt and EGFR expression in the JHU006 cell line (*P < 0.05). Akt was the only one of the four proteins examined to show a significant reduction in expression in the JHU020 cell line (*P < 0.05). The statistics represent the standard errors of the means from three separate experiments. The western blots are cropped in order to improve clarity.