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. 2020 Feb 11;30(3):256–268. doi: 10.1038/s41422-020-0281-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Profiles of the averaged H3K27ac (a) and H3K36me3 (b) tag density on the four subtypes of hotspot-associated H3K4me3 peaks at mid-zygotene. H3K27ac and H3K36me3 tag densities were calculated using ChIP-seq read coverage with 50-bp resolution. c The average number of NDRs per DSB ±2 kb at the sites of four subtypes of hotspot-associated H3K4me3 peaks. NDRs were defined in mid-zygotene spermatocytes. d Boxplots showing the chromatin accessibility level in five regions, early-forming H3K4me3 regions, persistent H3K4me3 regions, slow-turnover H3K4me3 regions, fast-turnover H3K4me3 regions, and whole-genome regions excluding these four cluster regions. Only regions with at least three GCH sites were considered.