Figure 4.

Effect of ryanodine receptor (RyR) variants on crawling changes with ageing. Crawling amplitudes, wavelengths and frequencies, and worm lengths, were extracted from 1 min long, 25 frames per second, video recordings of 20–30 individual 10-day old adults. Worm length (A), maximum amplitude versus wavelength (B) and maximum amplitude versus frequency (C) for RyR variant strains, labelled by the human variant they correspond to, along with the N2 wild type and the CB540 unc-68(e540) null mutant, were compared. Maximum amplitude and wavelength were normalized for worm length. Boxes indicate the median and interquartile range, with whiskers to the 10–90 percentile, outliers as dots, and + to indicate the mean (A). Mean and SEM are indicated, although SEM is very small for frequency and for normalized maximum amplitude and wavelength (B, C). The change in maximum amplitude and primary wavelength from young adult (hollow symbols) to old adult (solid symbols), is indicated by a straight line (B, C). Representative kymograms are presented for the N2 wild type and for hR2163H and hR2458H RyR variant strains for (D) young adults (YA) and (E) old adults (OA). Colour indicates degree of dorsal/ventral curvature (red/blue high curvature, green no curvature) at different positions along the body axis (0 anterior to 50 posterior) for each frame of the video recording. Individual frames where parameters could not be extracted are black.