Table 1.
Amino acid alignment of human RyR1 and C. elegans UNC-68 in the regions of the studied variants, and the C. elegans strain expressing the variant UNC-68.
| Human RyR1 variant (C. elegans variant) | Alignment | C. elegans strain | |
|---|---|---|---|
| R163C (R169C) |
RyR1 | ASKQRSEGEKVRVGDDIILVS | UL4239 |
| UNC-68 | ASKQRSEGEKVRVGDDVILVS | ||
| G341R (G350R) |
RyR1 | PPEIKYGESLCFVQHVASGLW | COP1879 |
| UNC-68 | NATIRYGETNAFIQHVKTQLW | ||
| R2163H (R2246H) |
RyR1 | LLECLGQIRSLLIVQMGPQEE | COP1883 |
| UNC-68 | FLVYLIQIRELLTVQFEHTEE | ||
| N2342S (N2441S) |
RyR1 | DFLRFAVFVNGESVEENANVV | UL4285 |
| UNC-68 | DFLRFCVWINGENVEENANLV | ||
| R2454H (R2560H) |
RyR1 | AGKGEALRIRAILRSLVPLED | COP1947 |
| UNC-68 | AGKGDSLRARAILRSLISLDD | ||
| R2458H (R2564H) |
RyR1 | AGKGEALRIRAILRSLVPLED | COP1944 |
| UNC-68 | AGKGDSLRARAILRSLISLDD | ||
| K3452Q (K3675Q) |
RyR1 | IYWSKSHNFKREEQNFVVQNE | COP1932 |
| UNC-68 | RIWSQSQHFKREELNYVAQFE | ||
| R4861H (R5021H) |
RyR1 | VVVYLYTVVAFNFFRKFY-NK | COP1950 |
| UNC-68 | VVVYLYTVIAFNFFRKFYVQE |
The residue changed in the variants is in bold and underlined. The UNC-68 residue numbering is based on isoform a.