Table 3.
Response of ryanodine receptor (RyR) variant strains, identified by the human variant they correspond to, and of the N2 wild type and the CB540 unc-68(e540) null mutant, to 1 mM levamisole in M9 buffer over 2 h.
| Time (min) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 10 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 | 120 | |
| Wild type | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| hR163C | 2/3 | 3/4 | 4 | 4 | 4 | 4 | 4 | 3/4 | 3 | 3 | 3 | 3 | 3 |
| hG341R | 1/2 | 2/3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| hR2163H | 2/3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| hN2342S | 1/2 | 3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0 | 0 |
| hR2454H | 2 | 3 | 3 | 3 | 3/4 | 3/4 | 3/4 | 3 | 3 | 3 | 0/3 | 0/3 | 0/3 |
| hR2458H | 1/2 | 3 | 3 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| hK3452Q | 1/2 | 3 | 3 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 3/4 | 3/4 | 3/4 |
| hR4861H | 2/3 | 3 | 3 | 3 | 0 | 0 | 0/3 | 0/3 | 0/3 | 0/3 | 3 | 3 | 0/3 |
| unc-68 null | 2/3 | 2/3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
No movement 0, No movement with attenuated movement 0/3, Swimming 1, Uncoordinated swimming 2, Attenuated movement 3, Kinking 4.