Figure 3.

Composition of the photosynthetic protein complexes in A. thaliana nourished with 5 mM ${\mathrm{NH}}_4^{+}$ or 5 mM ${\mathrm{NO}}_3^{-}$ (control) as the only source of nitrogen. (A) Identification of thylakoid protein complexes in Blue Native (BN)-polyacrylamide gel electrophoresis (PAGE)-gel. Thylakoid membranes were solubilized in 1% dodecyl maltoside (DDM), and the protein extracts containing 8.3 µg of chlorophyll were separated by electrophoresis. Bands with different letters quantified by densitometry indicate significant difference (p ≤ 0.05). (B) Patterns of thylakoid protein complexes in second dimension by 2D-PAGE. BN-PAGE strips were denatured and separated on sodium dodecyl sulfate (SDS)-Urea-PAGE and were silver-stained. Representative gels are shown out of n = 3−6 independent replicates.