Figure 7.

Export of reductants out of chloroplasts in A. thaliana nourished with 5 mM ${\mathrm{NH}}_4^{+}$ or 5 mM ${\mathrm{NO}}_3^{-}$ (control) as the only source of nitrogen. (A) Protein abundance of mitochondrial serine hydroxymethyltransferase (SHMT, 53 kDa); mitochondrial glycine decarboxylase complex H subunit (GDC-H, 16 kDa); and peroxisome hydroxypyruvate reductase (HPR, 43 kDa). Representative immunoblots are shown out of n = 3−4 independent replicates. (B) Capacity of NADP-malate dehydrogenase (NADP-MDH). (C) Transcript level of chloroplast NADP-MDH. The relative expression is set to 1 in WT plants grown with ${\mathrm{NO}}_3^{-}$ (control) for reference. Bars or bands with different letters indicate significant difference (p ≤ 0.05).