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. 2020 Mar 3;10:3927. doi: 10.1038/s41598-020-60802-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Collagen cleavage assay. Purified recombinant 62-kDa collagenase from Grimontia hollisae was incubated with type I (A), type II (B), type III (C), type IV (D), type V (E), and type VI (F) collagens at 30 °C. After the reaction was stopped by the addition of 1/4 volume of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer at each time point, each sample was analysed by SDS-PAGE using a 7.5% polyacrylamide gel. The uncropped gel is included in a Supplementary Information File.