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. 2020 Feb 26;11:321. doi: 10.3389/fimmu.2020.00321

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Copyright © 2020 Salomonsson, Dahlin, Ungerstedt and Hallgren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mouse MCp in the bone marrow express CCR1 transcripts and protein. (A) Lung MCp were identified as CD45⁺ Lin⁻ c-kit⁺ T1/ST2⁺ integrin β7^hi CD16/32⁺ cells. (B) MCp from naïve or influenza-infected (IAV) bone marrow (BM) or lungs were FACS-sorted, lysed and analyzed by qPCR for the mRNA expression of chemotactic receptors. CCR1 and CX₃CR1 mRNA were detected in the majority of the samples. The relative expression using RPL30 as the endogenous control is shown. All detected values from three independent experiments are shown. (C) Representative histograms from three experiments aimed at determining CCR1 and CX₃CR1 surface expression by bone marrow MCp from naïve mice or lung MCp from influenza (IAV) infected mice, compared with FMO control including isotype control staining.