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. 2020 Feb 26;11:321. doi: 10.3389/fimmu.2020.00321

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Copyright © 2020 Salomonsson, Dahlin, Ungerstedt and Hallgren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mouse MCp in the bone marrow, spleen and lung demonstrate surface expression of CCR5. (A,B) Bone marrow (BM) and spleen cells from naïve BALB/c mice were stained by fluorescently-labeled antibodies and analyzed by flow cytometry. (A) Representative histograms from three independent experiments demonstrating surface expression of CCR5 by BM MCp (B). Representative histograms of three independent experiments demonstrating surface expression of CCR5 by spleen MCp. (C) Isolated lung cells from PBS-injected or influenza-infected (IAV) mice were stained with fluorescently-conjugated antibodies and analyzed by flow cytometry. Representative histograms from three experiments with three-nine mice per group per experiment demonstrating surface expression of CCR5 by lung MCp.