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. 2020 Feb 26;11:321. doi: 10.3389/fimmu.2020.00321

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Copyright © 2020 Salomonsson, Dahlin, Ungerstedt and Hallgren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Human MCp in bone marrow and peripheral blood demonstrate surface expression of CCR1 and CCR5. Ficoll-separated cells from bone marrow (BM) and blood of five healthy donors were stained with fluorescently-conjugated antibodies and analyzed by flow cytometry. (A) MCp were identified as CD4⁻ CD8⁻ CD19⁻ CD14⁻ CD34^hi CD117⁺ FcεRI⁺ cells. (B) Representative histograms for CCR1 and CCR5 surface expression on blood and BM MCp. (C) The differential expression of CCR1 and CCR5 by MCp from blood and BM were compared and illustrated as a difference in gMFI. Each donor is labeled by a specific color. The bars show the mean ± SEM. ^**p < 0.01.