Table 2.
The mRNA expression of chemotactic receptors on sorted MCp from naïve bone marrow, influenza infected (IAV) bone marrow or lung.
| Naïve bone marrow | IAV bone marrow | IAV lung | |
|---|---|---|---|
| BLT1 | 36, –, 30 (2/3) | –, 29, 34, (2/3) | –, –, –, (0/3) |
| EP3 | –, –, –, (0/3) | –, 31, –, (1/3) | 32, 33, –, (2/3) |
| CXCR2 | –, –, –, (0/3) | –, 32, 35, (2/3) | 33, 33, –, (2/3) |
| CXCR3 | –, –, –, (0/3) | –, 37, –, (1/3) | 38, 36, – (2/3) |
| CXCR5 | –, –, –, (0/3) | –, 31, –, (1/3) | 33, 33, –, (2/3) |
| CX3CR1 | 26, 26, 36 (3/3) | –, 31, – (1/3) | 32, 26, – (2/3) |
| CCR1 | 26, 28, 28 (3/3) | 29, 28, 35 (3/3) | 33, 35, 25 (3/3) |
| CCR2 | –, –, –, (0/3) | 35, 32, 34, (3/3) | 34, 32, –, (2/3) |
| CCR3 | –, 28, –, (1/3) | –, 31, –, (1/3) | 34, 32, –, (2/3) |
| CCR4 | –, –, –, (0/3) | –, 33, –, (1/3) | 35, 34, –, (2/3) |
| CCR5 | –, –, –, (0/3) | –, 32, –, (1/3) | 34, 34, –, (2/3) |
| CCR6 | –, –, –, (0/3) | –, 32, –, (1/3) | –, 34, –, (1/3) |
| CCR7 | –, –, –, (0/3) | –, 32, –, (1/3) | 33, 33, –, (2/3) |
| CCR8 | –, –, –, (0/3) | –, 31, 34, (2/3) | 32, 33, –, (2/3) |
| CCR9 | –, –, –, (0/3) | –, 32, 35, (2/3) | 34, 33, –, (2/3) |
| RPL30 | 23, 24, 23 (3/3) | 23, 25, 20 (3/3) | 20, 19, 20 (3/3) |
| UBC | 29, –, –, (1/3) | –, 32, 34, (2/3) | 26, 33, – (2/3) |
| GAPDH | –, 30, 26 (2/3) | 27, 26, 27, (3/3) | 25, 24, 25 (3/3) |
The number of PCR cycles required to reach the threshold is shown for each experiment and target. The number of experiments with a detected signal is shown in the parenthesis. RPL30, UBC and GAPDH were used as endogenous controls.
The number in parenthesis indicate number of experiments with signal for the specific receptor. –, no signal.