Table 3.
Description of chemokine receptor detection antibody.
| Antibody target | Clone | Positive control | |
|---|---|---|---|
| Mouse | CX3CR1 | 528728 | CD115+ Gr-1− CD16/32hi splenocytes |
| CXCR2 | 242216 | CD11b+ splenocytes | |
| CXCR3 | CXCR3-173 | CD3+ splenocytes | |
| CXCR4 | 2B11/CXCR4 | CD4+ cells from thymus | |
| CXCR5 | SPRCL5 | CD19+ cells from peritoneal cavity | |
| CCR1 | 643854 | CD115+ Gr-1− splenocytes | |
| CCR2 | 475301 | CD11b+ splenocytes | |
| CCR3 | J073E5 | Gr-1+ splenocytes | |
| CCR5 | HM-CCR5 (7A4) | DX-5+ CD11b+ CD4− CD8− splenocytes | |
| CCR6 | 29-2L17 | B220+ splenocytes | |
| CCR7 | 4B12 | CD4+ splenocytes | |
| CCR9 | eBioCW-1.2 | CD4+ cells from thymus | |
| Human | CCR5 | 3A9 | CD14+ monocytes from bone marrow and blood |
| CCR1 | 53504 | CD14+ monocytes from bone marrow and blood |
Clone of detection antibody used for detection. Description of which cells and antibodies that were used to identify positive controls for each chemokine receptor.