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. 2020 Mar 3;11(3):167. doi: 10.1038/s41419-020-2356-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Bioinformatics analysis of potential miRNAs that target HMGA1P6, HMGA1 and HMGA2 3′UTR was conducted. b, c HMGA1P6, HMGA1 and HMGA2 expression were measured by qPCR after transfection of miRNA mimics or inhibitors. d–f Luciferase activity was tested after co-transfection of reporter plasmid containing HMGA1/2 3′UTR, HMGA1P6 wild type or mutant transcript and miRNA mimics. g Relative expression of HMGA1P6 and HMGA1/2 was measured in HEY cells after transfection of HMGA1/2 siRNAs. h Relative enrichment fold change of HMGA1P6 was analyzed in Ago2-RIPs compared to control IgG-RIPs. i Ago2 in biotinylated HMGA1P6 pulldown lysates in HEY cells was tested by western blot. j Matrigel invasion assay was performed in HEY cells transfected with different vectors for rescue experiment. Data are presented as means ± S.D. *p < 0.05, **p < 0.01.