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. 2020 Mar 3;10:3886. doi: 10.1038/s41598-020-60688-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcription factor combinations synergistically induce endothelial cell junction proteins expression and mRNA expression of EC barrier relevant genes. Immunocytochemistry with confocal imaging and ImageJ analysis of (a) CLDN5, (b) OCLN and (c) ZO1 (all in red) after 48 h treatment with combination of TFs. As EC barrier marker, VE-Cadherin was stained in green and nucleus with DAPI in blue. Scale bar represents 50 µm. (d) Relative mRNA expression of genes involved in barrier junction and transport across EC barrier after treatment with combination of TFs for 48 h. Columns are mean ± SD. All transduction were performed as 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.