Fig. 2. B cell-induced T cells possess immunosuppressive effects via IL-22.

a The schematic graph show that naive B cells sorted from CD45.1⁺ background B6 mice were cultured in the presence of α-IgM and α-CD40 for 2 days, then co-cultured with sorted naive T cells from CD45.1⁺ B6 mice under Th17 cell culture condition for 5 days. CD45.1⁺ T cells were then sorted and co-cultured with naive T and B cells from CD45.2⁺ B6 mice with or without anti-IL-22 antibodies for additional 5 days. b CD45.1⁺ T and CD45.2⁺ T cells were detected by flow cytometry. c IL-22R expression in CD45.2⁺ T and CD45.2⁺ B cells was analyzed by flow cytometry. d CD45.2⁺ T cells were labeled with BrdU, then cell proliferation was analyzed by flow cytometry. e The flow cytometric results of CD4⁺IFN-γ⁺ cells. f The flow cytometric results of CD4⁺TNF-α⁺ cells. g CD45.2⁺ B cells were labeled with BrdU, then analyzed by flow cytometry. h The flow cytometric results of CD19⁺IL-10⁺ cells. i The flow cytometric results of CD19⁺TGF-β⁺ cells. j IgM in supernatants was analyzed by ELISA. k IgG in supernatants was analyzed by ELISA. Results shown are representative of three independent experiments. *p < 0.05.