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. 2019 Dec 20;122(5):658–667. doi: 10.1038/s41416-019-0688-y

Fig. 1. Effect of HSP90 inhibition on mutant KIT signalling.

Fig. 1

a KIT gene mutation and effects of IM, TAS-116 and 17-AAG in IM-naïve and IM-resistant GIST cell lines. b Cell proliferation was determined by WST-8 assays at 72 h after incubation with TAS-116. Each value is represented as the mean ± SD. c Western blot analysis of HSP90, HSP70, pKIT/KIT, pAKT/AKT and pERK/ERK in GIST cell lysates at 12 h after IM, TAS-116 and 17-AAG treatments was performed. d Caspase-3/7 activity was determined using luminescence assays 24 h after incubation with 0.1 μM IM and 0.25–1.0 μM TAS-116.