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. 2020 Mar 3;10:3908. doi: 10.1038/s41598-020-60705-z

Table 1.

Primer used for Real time PCR.

mRNA Product size (bp) Annealing temperature (°C) Sequence
HPRT (mouse) 163 60–63

F: 5'-GTTGGATACAGGCCAGACTTTGTTG-3'

R: 5'-GATTCAACTTGCGCTCATCTTAGGC-3'

VEGF-A (mouse) 184 60

F: 5'-CATGGATGTCTACCAGCGAAG-3'

R: 5'-CATGGTGATGTTGCTCTCTGAC-3’

VEGF-C (mouse) 219 60

F: 5'-AGAACGTGTCCAAGAAATCAGC-3'

R: 5'-ATGTGGCCTTTTCCAATACG-3’

VEGF-D (mouse) 86 62.4

F: 5'-ATGGCGGCTAGGTGATTCC-3’

R: 5'-CCCTTCCTTTCTGAGTGCTG-3’

MCP-1 (mouse) 312 60

F: 5'- ACCACAGTCCATGCCATCAC -3'

R: 5'- TTGAGGTGGTTGTGGAAAAG -3'