Table 1.
mRNA | Product size (bp) | Annealing temperature (°C) | Sequence |
---|---|---|---|
HPRT (mouse) | 163 | 60–63 |
F: 5'-GTTGGATACAGGCCAGACTTTGTTG-3' R: 5'-GATTCAACTTGCGCTCATCTTAGGC-3' |
VEGF-A (mouse) | 184 | 60 |
F: 5'-CATGGATGTCTACCAGCGAAG-3' R: 5'-CATGGTGATGTTGCTCTCTGAC-3’ |
VEGF-C (mouse) | 219 | 60 |
F: 5'-AGAACGTGTCCAAGAAATCAGC-3' R: 5'-ATGTGGCCTTTTCCAATACG-3’ |
VEGF-D (mouse) | 86 | 62.4 |
F: 5'-ATGGCGGCTAGGTGATTCC-3’ R: 5'-CCCTTCCTTTCTGAGTGCTG-3’ |
MCP-1 (mouse) | 312 | 60 |
F: 5'- ACCACAGTCCATGCCATCAC -3' R: 5'- TTGAGGTGGTTGTGGAAAAG -3' |