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. 2019 Jul 1;33(4):547–555. doi: 10.5713/ajas.19.0173

Table 2.

The primers of the mRNAs and miRNAs for qualitative real-time polymerase chain reaction

Primer name Primer sequences Tm (°C) Length (bp)
DHCR24 F CAGAAATCCCACCCAGAGAG 58 210
DHCR24 R GACAGCCAACAGGCAGATAG
Bcl-2-F CTTTGCCGAGATGTCCAGC 60 197
Bcl-2-R TCCACAGGGCGATGTTGTC
Caspase-3-F TAACCCGAGTAAGAATGT 51 160
Caspase-3-R ATACCAGTTGAGGCAGAC
β-Actin F CTCGATCATGAAGTGCGACG 60 114
β-Actin R GTGATCTCCTTCTGCATCCTGTC
miR-26a GCTTCAAGTAATCCAGGATAGGCT
U6 snRNA GGCAAGGATGACACGCAAAT

DHCR24, 3β-hydroxysteroid-Δ24-reductase.