Upregulation of cleaved caspase-6 and cleaved caspase-9 in the inner medulla of 24-h bilateral ureteral obstruction (BUO) rats. A and B: representative immunoblots from whole inner medullas of 4-h BUO (+, n = 7) versus sham (−, n = 7) and 10-h BUO (+, n = 7) versus sham (−, n = 7) rats demonstrated that cleaved caspase-9 abundance did not significant differ from sham rats. D and E: cleaved caspase-6 was significant downregulated after 4-h BUO by 34% (unpaired t tests, *P < 0.05; D) but did not significant change compared with sham rats after 10-h BUO (E). C and F: densitometry analysis revealed upregulations of cleaved caspase-6 and cleaved caspase-9 by 48% and 49%, respectively, in 24-h BUO (+, n = 7) compared with sham (−, n = 7) rats (unpaired t tests, *P < 0.05, ***P < 0.005).