Figure EV4. Uc.291 protein microarray.

1. Schematic depicting high‐throughput analysis performed to identify uc.291 protein interactome.
2. Protein array analysis showing the top 20 chromatin‐related candidates; see also Appendix Table S2.
3. Reproducibility between the two human recombinant protein arrays spotted with 50,000 different proteins. (Pearson's correlation = 0.76; F‐B > 0).
4. Area under ROC curve (AUC) to assess Global Score 34 ability to separate proteins interacting with uc.291 from those that are non‐interacting. The F‐B score was employed to select different groups of high‐affinity (top ranked) and low‐affinity (bottom ranked) interactions reported in the array: (1) RBP, (2) chromatin‐related and (3) unfiltered (i.e. without selecting by category). In all cases, Global Score performances increase from poor signal (top/bottom 200) to strong signal (top/bottom 10) interactions, showing AUCs that raise from 0.60 to 0.80.