Table 2.
Plasmids used in this study
| Plasmid/epitope tag | Gene/mutation | Reference |
|---|---|---|
| pDONOR223‐hATG2A | Human ATG2A | This study |
| pDONOR223‐hATG2B | Human ATG2B | This study |
| (iTAP) pMSCV‐Flag‐HA‐ATG2A | ATG2A‐WT | This study |
| (iTAP) pMSCV‐Flag‐HA‐ATG2A‐mLIR | ATG2A aa1,362–1,365 FCIL/AAAA | This study |
| (iTAP) pMSCV‐Flag‐HA‐ATG2A‐mYFS | ATG2A aa1,395–1,397 YFS/AAA | This study |
| pDEST‐CMV‐Myc‐ATG2A‐WT | ATG2A‐WT | This study |
| pDEST‐CMV‐Myc‐ATG2A‐mLIR#1 | ATG2A aa536–539 YTEI/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2A‐mLIR#2 | ATG2A aa926–929 FSTL/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2A‐mLIR#3 | ATG2A aa981–984 YFCL/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2A‐mLIR#4 | ATG2A aa1,092–1,095 FLDV/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2A‐mLIR #5 | ATG2A aa1,362–1,365 FCIL/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2B‐WT | ATG2B‐WT | This study |
| pDEST‐CMV‐Myc‐ATG2B‐mLIR#1 | ATG2B aa845–848 WPRI/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2B‐mLIR#3 | ATG2B aa979–982 FENI/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2B‐mLIR#4 | ATG2B aa1,026–1,029 FSTV/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2B‐mLIR#5 | ATG2B aa1,306–1,309 YVRV/AAAA | This study |
| pDEST‐CMV‐Myc‐ATG2B‐mLIR#6 | ATG2B aa1,491–1,494 FCIL/AAAA | This study |
| pGEX‐4T1 alone | GST only | 5 |
| pGEX‐4T1‐LC3A‐ΔG | MAP1LC3A deletion of c‐term Gly | 5 |
| pGEX‐4T1‐LC3B‐ΔG | MAP1LC3B deletion of c‐term Gly | 5 |
| pGEX‐4T1‐LC3C‐ΔG | MAP1LC3C deletion of c‐term Gly | 5 |
| pGEX‐4T1‐GABARAP‐ΔG | GABARAP deletion of c‐term Gly | 5 |
| pGEX‐4T1‐GABARAP‐L1‐ΔG | GABARAP‐L1 deletion of c‐term Gly | 5 |
| pGEX‐4T1‐GABARAP L2‐ΔG | GABARAP‐L2 deletion of c‐term Gly | 5 |
| pEGFP‐C1 empty | GFP only | 5 |
| pEGFP‐C1‐LC3A | GFP‐tagged MAP1LC3A | 5 |
| pEGFP‐C1‐LC3B | GFP‐tagged MAP1LC3B | 5 |
| pEGFP‐C1‐LC3C | GFP‐tagged MAP1LC3C | 5 |
| pEGFP‐C1‐GABARAP | GFP‐tagged GABARAP | 5 |
| pEGFP‐C1‐GABARAP‐L1 | GFP‐tagged GABARAP‐L1 | 5 |
| pEGFP‐C1‐GABARAP‐L2 | GFP‐tagged GABARAP‐L2 (GATE‐16) | 5 |
| pmCherry‐C1‐GABARAP | mCherry‐tagged GABARAP | This study |
| pDEST‐mCherry‐WIPI4 | mCherry‐tagged WIPI4 (WDR45) | This study |
| pDEST‐EGFP‐WIPI4 | GFP‐tagged WIPI4 (WDR45) | This study |
| pMRXIP‐GFP‐Stx17 WT | GFP‐tagged Syntaxin 17 | 51 |